Title :
Effects of high field DC pulses on the viability of Lactobacillus brevis
Author :
Jayaram, Shesha ; Castle, G.S.P. ; Margaritis, A.
Author_Institution :
Fac. of Eng. Sci., Univ. of Western Ontario, London, Ont., Canada
Abstract :
Inactivation of Lactobacillus brevis cells in suspensions of phosphate buffer solutions of different conductivities (170 μS/cm to 2230 μS/cm) using electric pulse application has been investigated. Survivability as a function of pulse application number has shown a logarithmic death for all test conditions. A maximum reduction in survivability was obtained in low-conductivity liquid medium. The pulse energy influences the survival rate of L. brevis cells by altering the liquid temperature during pulse discharge; however, the direct lethal effect of heating was insignificant as the maximum temperature rise was only 22°C upon application of 600 pulses of 8 W-s/pulse
Keywords :
bioelectric phenomena; biological effects of fields; cellular transport and dynamics; Lactobacillus brevis; direct lethal effect of heating; high field DC pulses; logarithmic death; low-conductivity liquid medium; microorganisms; phosphate buffer solutions; pulse energy; sterilization; survivability; survival rate; suspensions; viability; Conductivity; Current; Electrochemical processes; Electrodes; Heat treatment; Microorganisms; Space vector pulse width modulation; Suspensions; Temperature; Testing;
Conference_Titel :
Electrical Insulation and Dielectric Phenomena, 1992. Annual Report. Conference on
Conference_Location :
Victoria, BC
Print_ISBN :
0-7803-0565-5
DOI :
10.1109/CEIDP.1992.283161
