Effects of high field DC pulses on the viability of Lactobacillus brevis

Author

Jayaram, Shesha ; Castle, G.S.P. ; Margaritis, A.

Author_Institution

Fac. of Eng. Sci., Univ. of Western Ontario, London, Ont., Canada

fYear

1992

Firstpage

529

Lastpage

536

Abstract

Inactivation of Lactobacillus brevis cells in suspensions of phosphate buffer solutions of different conductivities (170 μS/cm to 2230 μS/cm) using electric pulse application has been investigated. Survivability as a function of pulse application number has shown a logarithmic death for all test conditions. A maximum reduction in survivability was obtained in low-conductivity liquid medium. The pulse energy influences the survival rate of L. brevis cells by altering the liquid temperature during pulse discharge; however, the direct lethal effect of heating was insignificant as the maximum temperature rise was only 22°C upon application of 600 pulses of 8 W-s/pulse

Keywords

bioelectric phenomena; biological effects of fields; cellular transport and dynamics; Lactobacillus brevis; direct lethal effect of heating; high field DC pulses; logarithmic death; low-conductivity liquid medium; microorganisms; phosphate buffer solutions; pulse energy; sterilization; survivability; survival rate; suspensions; viability; Conductivity; Current; Electrochemical processes; Electrodes; Heat treatment; Microorganisms; Space vector pulse width modulation; Suspensions; Temperature; Testing;

fLanguage

English

Publisher

ieee

Conference_Titel

Electrical Insulation and Dielectric Phenomena, 1992. Annual Report. Conference on

Conference_Location

Victoria, BC

Print_ISBN

0-7803-0565-5

Type

conf

DOI

10.1109/CEIDP.1992.283161

Filename

283161