A micro system using dielectrophoresis and electrical impedance spectroscopy for cell manipulation and analysis

Author

Mohanty, S.K. ; Ravula, S.K. ; Engisch, K.L. ; Frazier, A.B.

Author_Institution

Sch. of Electr. & Comput. Eng., Georgia Inst. of Technol., Atlanta, GA, USA

Volume

2

fYear

2003

Firstpage

1055

Abstract

Single cell manipulation and analysis using dielectrophoresis (DEP) and micro electrical impedance spectroscopy (/spl mu/-EIS) has been demonstrated on bovine chromaffin cells and red blood cells. Cells were manipulated and analyzed on a micro scale electrophysiological analysis system fabricated using micromachining (e.g. MEMS) technologies. Cells were injected into a microreservoir and collected for /spl mu/-EIS analysis using vacuum and DEP. Impedance measurements were taken over a frequency range of 40 Hz to 3.0 MHz. The data obtained for the cells was compared to data obtained for air, and phosphate buffered solution. Data for chromaffin cells was analyzed further, assessing cell size and calcium ion channel activity in relation to impedance spectroscopy.

Keywords

blood; calcium; cellular biophysics; electric impedance measurement; electrophoresis; microfluidics; micromachining; 40 Hz to 3 MHz; Ca; MEMS; bovine chromaffin cells; calcium ion channel activity; cell manipulation; cell size; dielectrophoresis; electrical impedance spectroscopy; micromachining; microreservoir; microscale electrophysiological analysis system; microsystem; red blood cells; Bovine; Calcium; Cells (biology); Dielectrophoresis; Electrochemical impedance spectroscopy; Frequency; Impedance measurement; Micromachining; Micromechanical devices; Red blood cells;

fLanguage

English

Publisher

ieee

Conference_Titel

TRANSDUCERS, Solid-State Sensors, Actuators and Microsystems, 12th International Conference on, 2003

Conference_Location

Boston, MA, USA

Print_ISBN

0-7803-7731-1

Type

conf

DOI

10.1109/SENSOR.2003.1216950

Filename

1216950