Autonomous microneedle system for biochemical analysis

Author

Chandrasekaran, S. ; Mohanty, S.K. ; Frazier, A.B.

Author_Institution

Sch. of Electr. & Comput. Eng., Georgia Inst. of Technol., Atlanta, GA, USA

Volume

2

fYear

2003

Firstpage

1442

Abstract

Autonomous microneedle system with bioluminescent-based sensing capabilities are presented. The analysis systems consists of a microneedle for sample introduction, a stereolithography defined fluid interface containing the analysis reservoir, and a detector. Enzyme catalyzed luciferin-luciferase biochemical reactions driven by adenosine triphosphate were used as the basic transduction mechanism. The reaction chemistry can be biochemically coupled to metabolic reactions for sensing of a variety of metabolites. Two detection mechanisms were studied as methods for quantification of the luminescent output generated from the reaction, the first based on lossy, planar polycarbonate waveguides and the second based on semiconductor photodetectors. The total analysis systems was fabricated and characterized for an ATP concentration range from 2.0 mM to 7.0 mM.

Keywords

biochemistry; bioluminescence; biosensors; inorganic compounds; micromechanical devices; molecular biophysics; optical planar waveguides; photodetectors; stereolithography; ATP concentration; adenosine triphosphate; analysis reservoir; autonomous microneedle; biochemical coupling; biochemistry; bioluminescent-based sensing capabilities; detector; enzyme catalyzed luciferin-luciferase biochemical reactions; fluid interface; planar polycarbonate waveguides; reaction chemistry; semiconductor photodetectors; stereolithography; transduction mechanism; Biochemical analysis; Biochemistry; Bioluminescence; Chemistry; Detectors; Photodetectors; Planar waveguides; Reservoirs; Semiconductor waveguides; Stereolithography;

fLanguage

English

Publisher

ieee

Conference_Titel

TRANSDUCERS, Solid-State Sensors, Actuators and Microsystems, 12th International Conference on, 2003

Conference_Location

Boston, MA, USA

Print_ISBN

0-7803-7731-1

Type

conf

DOI

10.1109/SENSOR.2003.1217047

Filename

1217047