Title :
Pulsed laser triggered high speed fluorescence activated microfluidic switch
Author :
Wu, Ting-Hsiang ; Chen, Yue ; Park, Sung-Young ; Hur, Soojung Claire ; Carlo, Dino Di ; Chiou, Eric Pei-Yu
Author_Institution :
Dept. of Electr. Eng., Univ. of California at Los Angeles (UCLA), Los Angeles, CA, USA
Abstract :
We report a high speed fluorescence activated microfluidic switch capable of achieving a switching time of 50 μsec with a detection efficiency of 86.6% and a switching efficiency of 86.5% at a particle flow speed of ~0.7 m/s. The switching mechanism is realized by exciting dynamic vapor bubbles with focused laser pulses in a microfluidic PDMS channel. The explosive bubble expansion generates fast fluid flows which are directed into a neighboring particle channel to switch the particle flow. Fluorescence activated switching of 10 micron polystyrene microspheres in a Y channel has been demonstrated. This ultrafast laser triggered switching mechanism has the potential to advance the sorting speed of the state-of-the-art microscale fluorescent activated cell sorting devices.
Keywords :
channel flow; fluorescence; high-speed optical techniques; micro-optics; microfluidics; optical polymers; optical switches; cell sorting devices; explosive bubble expansion; fluid flows; focused laser pulses; microfluidic PDMS channel; microfluidic switch; particle flow speed; polystyrene microspheres; pulsed laser triggered high speed fluorescence activation; switching efficiency; vapor bubbles; Fluorescence; Lenses; Microfluidics; Optical switches; Semiconductor lasers;
Conference_Titel :
Optical MEMS and Nanophotonics (OPT MEMS), 2010 International Conference on
Conference_Location :
Sapporo
Print_ISBN :
978-1-4244-8926-8
Electronic_ISBN :
978-1-4244-8925-1
DOI :
10.1109/OMEMS.2010.5672192
