Coherent raman fast spectral microscopy

Stimulated Raman scattering (SRS) microscopy is a label-free biomedical 3-D imaging technique suggested recently. The SRS microscopy offers various advantages, such as the accessibility to the vibrational spectrum, quantitative image contrast. We have developed a hyper-spectral SRS microscope system with rapid spectral tunability. A series of SRS images with a different vibrational content can be acquired in a frame-by-frame manner at up to the standard video rate. We employed multivariable analysis techniques to these hyperspectral images. The results of label-free 3-D imaging of biomedical samples and those of efficient and recognizable method of data reduction are presented.