Title :
Highly selective and sensitive DNA measurement by microelectrical impedance spectroscopy
Author :
Lee, Sang Wook ; Yamamoto, Takatoki ; Fujii, Teruo
Author_Institution :
Inst. of Ind. Sci., Tokyo Univ., Meguro, Japan
Abstract :
A novel μ-EIS (microelectrical impedance spectroscopy) device for label free analysis of DNA are developed and experimentally demonstrated. The feature of μ-EIS system is not only detecting DNA without any labelling, but also controlling the conformation that would enhance the EIS signal. The μ-EIS system is fully integrated with a microfluidic network made of polydimethylsiloxane (PDMS), a micromeasurement electrode and a micropump in order to conduct a semiautomated measurement controlled by an external PC system. The micro EIS system is capable of detecting the DNA concentration of a few nM in aqueous solution of a few pl in volume by virtue of the conformation enhanced impedance response. As a first demonstration of conformational change induced DNA analysis, the frequency and the electric field strength dependence of various length and concentrations of DNA were evaluated.
Keywords :
DNA; biomedical imaging; biomedical measurement; biosensors; electric impedance imaging; microelectrodes; microfluidics; micropumps; microsensors; molecular biophysics; DNA concentration; microelectrical impedance spectroscopy; microfluidic network; micromeasurement electrode; micropump; polydimethylsiloxane; Control systems; DNA; Electric variables measurement; Electrochemical impedance spectroscopy; Electrodes; Frequency; Glass; Impedance measurement; Microfluidics; Micropumps;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems, 2005. Digest of Technical Papers. TRANSDUCERS '05. The 13th International Conference on
Print_ISBN :
0-7803-8994-8
DOI :
10.1109/SENSOR.2005.1497322
