An electrically active microneedle array for electroporation of skin for gene delivery

Author

Choi, Seong-O ; Park, Jung-Hwan ; Choi, Yoonsu ; Kim, Yeuchun ; Gill, Harvinder S. ; Yoon, Yong-Kyu ; Prausnitz, Mark R. ; Allen, Mark G.

Author_Institution

Sch. of Electr. & Comput. Eng., Georgia Inst. of Technol., Atlanta, GA, USA

Volume

2

fYear

2005

Firstpage

1513

Abstract

We have designed and fabricated a microneedle array with electrical functionality with the final goal of electroporating skin´s epidermal cells to increase their transfection by DNA vaccines. The microneedle array was made of polymethylmethacrylate (PMMA) by micromolding technology from a master PDMS mold, followed by metal deposition, patterning using laser ablation, and electrodeposition. This microneedle array possessed sufficient mechanical strength to penetrate human skin in vivo and was also able to electroporate red blood cells as an in vitro model to demonstrate cell membrane permeabilization.

Keywords

DNA; drug delivery systems; electrodeposition; genetics; laser ablation; microfluidics; skin; DNA vaccines; PDMS mold; cell membrane permeabilization; electrical function; electrically active microneedle array; electrodeposition; gene delivery; human skin; laser ablation; mechanical strength; metal deposition; micromolding technology; polymethylmethacrylate; red blood cells; skin electroporation; skin epidermal cell; DNA; Epidermis; Humans; In vivo; Laser ablation; Laser modes; Optical arrays; Red blood cells; Skin; Vaccines;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems, 2005. Digest of Technical Papers. TRANSDUCERS '05. The 13th International Conference on

Print_ISBN

0-7803-8994-8

Type

conf

DOI

10.1109/SENSOR.2005.1497371

Filename

1497371