Title :
An AFM Method for in situ Probing Membrane Proteins under Physiological Condition
Author :
Li, Guangyong ; Xi, Ning ; Chan, Ho-Yin ; Wang, Donna H.
Author_Institution :
Department of Electrical and Computer Engineering, Michigan State University, East Lansing, Michigan 48824, Email: liguangyegr.msu.edu
Abstract :
Although membrane proteins are the main drug targets, the study of cell membrane proteins in situ at the molecular level is difficult due to the current technical limitations. The recent development of atomic force microscopy (AFM) opens a new avenue to study the functionalities of cell membrane proteins. In this paper, techniques involving the use of AFM to probe biomolecules are introduced. The techniques for characterizing specific single receptor using the functionalized AFM tip is discussed. An example of probing angiotensin II type 1 (AT1) receptor by functionalized AFM tip is also given in this paper.
Keywords :
Atomic force microscopy; Biomembranes; Cells (biology); High-resolution imaging; Interference; Molecular biophysics; Polymers; Probes; Protein engineering; Surface topography;
Conference_Titel :
Nanotechnology, 2006. IEEE-NANO 2006. Sixth IEEE Conference on
Print_ISBN :
1-4244-0077-5
DOI :
10.1109/NANO.2006.247602
