Suitability of Viability Assays for Testing Biological Effects of Coated Superparamagnetic Nanoparticles

Author

Bähring, Franziska ; Schlenk, Florian ; Wotschadlo, Jana ; Buske, Norbert ; Liebert, Tim ; Bergemann, Christian ; Heinze, Thomas ; Hochhaus, Andreas ; Fischer, Dagmar ; Clement, Joachim H.

Author_Institution

Dept. for Hematology & Oncology, Jena Univ. Hosp., Jena, Germany

Volume

49

Issue

1

fYear

2013

fDate

Jan. 2013

Firstpage

383

Lastpage

388

Abstract

The aim of this study was to establish an in vitro model test system for the analysis of cellular effects of nanoparticles on endothelial cells using the human brain microvascular endothelial cell line HBMEC, a representative of the blood-brain barrier. At first we defined a toxic end point by using superparamagnetic iron oxide nanoparticles coated with cationic substances (polyethylenimine, chitosan, diethylamine ethyl). To study the toxic effects of nanoparticles we evaluated three different viability assays and one cytotoxicity assay for their suitability. We could show that a luminescence-based assay is most promising for an accurate testing, because it is reproducible and unaffected by assay conditions and reagents.

Keywords

blood; brain; cellular biophysics; nanobiotechnology; nanoparticles; toxicology; HBMEC; biological effect; blood-brain barrier; cellular effect; chitosan; coated superparamagnetic nanoparticle; cytotoxicity assay; diethylamine ethyl; human brain microvascular endothelial cell line; luminescence based assay; polyethylenimine; suitability; superparamagnetic iron oxide; viability assay; Bars; In vitro; Nanobioscience; Nanomaterials; Nanoparticles; Testing; Iron oxide; superparamagnetic nanoparticle; viability assay;

fLanguage

English

Journal_Title

Magnetics, IEEE Transactions on

Publisher

ieee

ISSN

0018-9464

Type

jour

DOI

10.1109/TMAG.2012.2222635

Filename

6392350