An ISFET based analogue ratiometric method for DNA methylation detection

Author

Kalofonou, Melpomeni ; Toumazou, Christofer

Author_Institution

Dept. of Electr. & Electron. Eng., Inst. of Biomed. Eng., London, UK

fYear

2014

fDate

1-5 June 2014

Firstpage

1832

Lastpage

1835

Abstract

This paper presents the concept of a ratiometric approach for DNA methylation detection using the “Methylation Cell” for the indication of epigenetic abnormalities related to cancer. The “Methylation Cell” allows real-time detection of pH signals resulting from DNA based reactions using ISFET sensors, performing continuous computation of the ratio of DNA methylation, giving a discrete output signal when DNA methylation exceeds a certain predefined percentage. Fabricated in a typical 0.35μm CMOS process, it uses current-mode translinear circuits to perform computation in low power. Experimental results are presented, demonstrating its capabilities through an integration in a Lab-on-Chip (LoC) set-up using a microfluidic assembly.

Keywords

DNA; bioMEMS; biochemistry; cancer; cellular biophysics; electrochemical sensors; genetics; ion sensitive field effect transistors; lab-on-a-chip; microfluidics; microsensors; pH; CMOS process; DNA based reactions; DNA methylation detection; ISFET based analogue ratiometric method; ISFET sensors; Lab-on-Chip set-up; LoC; Methylation Cell; cancer; continuous computation; current-mode translinear circuits; discrete output signal; epigenetic abnormalities; low power computation; microfluidic assembly; pH signals; real-time detection; CMOS integrated circuits; Cancer; Chemical sensors; Chemicals; DNA; Sensors; DNA methylation; ISFET; Lab-on-Chip; translinear;

fLanguage

English

Publisher

ieee

Conference_Titel

Circuits and Systems (ISCAS), 2014 IEEE International Symposium on

Conference_Location

Melbourne VIC

Print_ISBN

978-1-4799-3431-7

Type

conf

DOI

10.1109/ISCAS.2014.6865514

Filename

6865514