Title :
Long period fiber grating biosensor for the detection of drug resistant bacteria: The “OPTObacteria” project
Author :
Consales, M. ; Quero, G. ; Zuppolini, S. ; Sansone, L. ; Borriello, A. ; Giordano, M. ; Venturelli, A. ; Costi, M.P. ; Santucci, M. ; Cusano, A.
Author_Institution :
Dept. of Eng., Univ. of Sannio, Benevento, Italy
Abstract :
We report on the development of a multilayer coated reflection-type long period fiber grating (LPG) biosensor, useful for the detection of antibiotic resistance bacteria. A standard LPG is first transformed in a more practical probe working in reflection mode, then it is coated by a primary layer of aPS and a secondary layer of PMMA in order to increase its surrounding refractive index sensitivity and at the same time provide the necessary conditions for a correct bio-functionalization. Standard linkage chemistry has been applied to anchor the bioreceptors on the probe surface. We show some preliminary results demonstrating the capability of our LPG biosensor to successfully monitor all the biological steps of the biomolecular experiments, including β-lactamase binding detection tests.
Keywords :
biomedical optical imaging; biosensors; drugs; fibre optic sensors; microorganisms; molecular biophysics; refractive index; β-lactamase binding detection tests; LPG biosensor; OPTObacteria project; PMMA; aPS; antibiotic resistance bacteria; bio-functionalization; biomolecular experiments; bioreceptors; drug resistant bacteria detection; long period fiber grating biosensor; multilayer coated reflection-type long period fiber grating; probe surface; reflection mode; standard linkage chemistry; surrounding refractive index sensitivity; Biosensors; Immune system; Optical buffering; Optical fiber sensors; Optical fibers; Probes; Sensitivity; β-lactamase detection; Long period fiber grating (LPGs); biomolecular sensing; reflection-type LPGs;
Conference_Titel :
Photonics Conference, 2014 Third Mediterranean
Conference_Location :
Trani
DOI :
10.1109/MePhoCo.2014.6866453
