Title :
On-chip detection of clinical Ebola virus RNA using specific DNA binding technique
Author :
Cai, H. ; Parks, J.W. ; Carrion, R. ; Zempoaltecatl, L. ; Patterson, J.L. ; Hawkins, A.R. ; Schmidt, Heidemarie
Author_Institution :
Sch. of Eng., Univ. of California, Santa Cruz, Santa Cruz, CA, USA
Abstract :
Clinical Ebola RNAs are detected with virus concentration down to 6.25 × 104 pfu/ml on an optofluidic chip. Longer complementary pull-down DNA sequences provide better isolation and higher signals.
Keywords :
RNA; biochemistry; cellular biophysics; microorganisms; molecular biophysics; molecular configurations; DNA binding technique; DNA sequences; on-chip clinical Ebola virus RNA detection; optofluidic chip; virus concentration; Biomedical optical imaging; DNA; Fluorescence; Magnetic liquids; Optical mixing; RNA; System-on-chip;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2014 Conference on
Conference_Location :
San Jose, CA
