Title :
Three-dimensional cell culture on microscaffolds with spatially resolved surface chemistry
Author :
Richter, Benjamin ; Pauloehrl, Thomas ; Kaschke, Johannes ; Fischer, J. ; Greiner, Alexandra M. ; Wegener, Martin ; Delaittre, Guillaume ; Barner-Kowollik, Christopher ; Bastmeyer, Martin
Author_Institution :
Cell- & Neurobiol., Karlsruhe Inst. of Technol., Karlsruhe, Germany
Abstract :
To spatially control protein-binding and cell-attachment in three dimensions (3D) we employ a two-photon-triggered cycloaddition of functional (e.g. biotinylated) dienophiles on the surface of 3D microscaffolds, which have been silanized with photoactivatable diens (photoenol).
Keywords :
biochemistry; cellular biophysics; molecular biophysics; proteins; surface chemistry; tissue engineering; 3D microscaffold surface; biotinylated dienophiles; cell-attachment; functional dienophiles; photoactivatable diens; photoenol; protein-binding; spatial resolved surface chemistry; three-dimensional cell culture; two-photon-triggered cycloaddition; Adhesives; Optical surface waves; Proteins; Resists; Three-dimensional displays;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2014 Conference on
Conference_Location :
San Jose, CA
