Title :
Enhanced spatial resolution in confocal laser microscopy by subtractive imaging using vector beams
Author :
Segawa, Susumu ; Kozawa, Yusuke ; Sato, Seiki
Author_Institution :
Inst. of Multidiscipl. Res. for Adv. Mater., Tohoku Univ., Sendai, Japan
Abstract :
The enhanced spatial resolution in confocal laser microscopy is demonstrated by subtracting two confocal images acquired by radially and azimuthally polarized beams. The effect of the side-lobe emerging in the subtraction processes is also discussed.
Keywords :
bio-optics; biological techniques; light polarisation; optical microscopy; azimuthally polarized beams; confocal laser microscopy; enhanced spatial resolution; radially polarized beams; subtractive imaging; vector beams; Fluorescence; Laser beams; Laser excitation; Laser theory; Microscopy; Spatial resolution;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2014 Conference on
Conference_Location :
San Jose, CA
