Effects of scattering particles´ concentration in laser speckle contrast imaging

As a powerful tool for imaging blood flow, laser speckle contrast imaging (LSCI) has been already applied in clinical diagnosis and pre-clinical researches. In LSCI, the contrast image provides the two-dimensional blood flow distribution information with penetration depth less than 500 μm. Over the past decade, spatial, temporal and spatiotemporal methods had been developed to obtain contrast image with high spatial and temporal resolution. However, the effects of scattering particles concentration are lack of investigation. In this study, we made a detailed study on this parameter. A series of in-vitro simulation experiments were conducted to investigate the effects of concentrations. The results showed that the traditional theory was unavailable when the concentration of scattering particles is low.