Development of a cell orientation control system for mouse embryo using electro-rotation

In this paper, a mouse embryo cell orientation control system using electro rotation is presented. Specifically, the electrodes hosting the mouse embryo was designed and fabricated using MEMS fabrication techniques. A vision tracking system was designed and tested to track the 2-D orientation of the mouse embryo. A two channel function generator was used to generate the phase-shifted voltages to generate the rotating electric field at the center of the electrodes. Multiple designs of the electrodes were proposed, and FEA simulations were conducted to investigate the electric field strength created by these electrodes when phase-shifted voltages were applied. Overall, the system is designed to control the orientation of the mouse embryo under an inverted microscope.