Circulatory culture system for elastic fiber development of tissue-engineered blood vessels

We proposed a fabrication technique of tubular tissues as an artery and the circulatory culture system for evaluating the development mechanisms of them. The tubular tissues were 2-6 mm in diameter, using a poly (L-lactide-co-ipusironn-caprolactone) (PLCL) scaffold. They consisted of the cell membranes and the PLCL scaffolds. Moreover, these tubular tissues had a mechanical property of artery because of the PLCL scaffolds. We successfully integrated them to the circulatory culture system. After that, we can evaluate the elastic fiber development of the tubular tissues with the mechanical stimulus utilizing the developed circulatory culture system.