Dielectrophoresis study of electroporation effects on Chinese hamster ovary cells

Author

Salimi, E. ; Braasch, Katrin ; Butler, Mairead ; Thomson, David J. ; Bridges, Greg E.

Author_Institution

Dept. of Electr. & Comput. Eng., Univ. of Manitoba, Winnipeg, MB, Canada

fYear

2014

fDate

13-16 July 2014

Firstpage

1

Lastpage

2

Abstract

Electroporation affects the dielectric properties of cells by creating pores in their membrane which allow transport of ions. Dielectrophoresis (DEP) is a powerful technique to investigate electroporation of single biological cells. Here we study the frequency dependent response of Chinese hamster ovary (CHO) cells and investigate the effect of different cell parameters on the DEP spectra. The results suggest low frequency DEP application (less than 200 kHz) for investigating the cell membrane conductivity, mid frequency DEP application (1-30 MH) for investigating the cell cytoplasm conductivity, and high frequency DEP application (greater than 100 MHz) for investigating cell cytoplasm permittivity.

Keywords

biological techniques; cellular biophysics; electrical conductivity; electrophoresis; permittivity; CHO cells; Chinese hamster ovary cells; DEP spectra; biological cells; cell cytoplasm conductivity; cell cytoplasm permittivity; cell membrane conductivity; dielectric properties; dielectrophoresis study; electroporation effects; frequency 1 MHz to 30 MHz; frequency dependent response; ion transport; mid frequency DEP; Biomembranes; Cells (biology); Conductivity; Dielectrophoresis; Force; Permittivity;

fLanguage

English

Publisher

ieee

Conference_Titel

Antenna Technology and Applied Electromagnetics (ANTEM), 2014 16th International Symposium on

Conference_Location

Victoria, BC

Type

conf

DOI

10.1109/ANTEM.2014.6887659

Filename

6887659