Foil-based BioMEMS for electrochemical capillary immunoassay for hepatitis serology

Author

Moser, Isabella ; Enderle, Barbara ; Kohn, Cordula ; Jobst, Gerhard ; Urban, Gerald

Author_Institution

Albert-Ludwigs-Univ., Freiburg, Germany

Volume

2

fYear

2005

fDate

5-9 June 2005

Firstpage

1649

Abstract

A foil-based BioMEMS for electrochemical capillary immunoassay for hepatitis serology is presented. Immobilization of the hepatitis antigen utilizing capillary forces and special stopping barriers in foil based microsystems and the application of it in fast immunoassays will be shown. The measurement starts with the addition of the antibody containing human serum sample. Subsequently the signal antibody labelled with glucose oxidase binds to the hepatitis B antibody and converts glucose to hydrogen peroxide, which is oxidized at the electrode in the measurement cell. The assay is very sensitive and designed to differentiate between hepatitis B negative (below 10 U/l) and positive serum. The present limit of detection of immunoassays in the BioMEMS is 20 atto-moles.

Keywords

biochemistry; biological techniques; biosensors; electrochemical electrodes; microelectrodes; microsensors; sugar; capillary forces; electrochemical capillary immunoassay; electrochemical detection; foil-based bioMEMS; foil-based microsystems; glucose oxidase; hepatitis B antibody; hepatitis B serology; hepatitis antigen; human serum sample; hydrogen peroxide; immunoassays detection; signal antibody; stopping barriers; Biosensors; Electrodes; Humans; Immune system; Liver diseases; Platinum; Resists; Silver; Sugar; Titanium;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems, 2005. Digest of Technical Papers. TRANSDUCERS '05. The 13th International Conference on

Print_ISBN

0-7803-8994-8

Type

conf

DOI

10.1109/SENSOR.2005.1497405

Filename

1497405