Title :
Digital imaging fluorescence microscopy: analytical purpose and low light level intensity
Author :
Salmon, J.M. ; Vigo, J. ; Lahmy, S. ; Viallet, P.
Author_Institution :
Perpignan Univ., France
Abstract :
It is shown that the problems encountered in the use of numerical analysis on digitized images obtained from low-light-level fluorescence imaging using intensified TV cameras to quantify fluorescence intensities can be overcome by an appropriate treatment of the digitized image. A protocol that corrects for the dark signal and pixel-to-pixel response variations, which are the sources of the problem, is presented. The efficiency of the protocol is exemplified by the evaluation of nuclear fluorescence intensities of 300 3T3 fibroblasts of the same population sample
Keywords :
biological techniques and instruments; biology computing; cellular biophysics; fluorescence; optical microscopy; spectrochemical analysis; 3T3 fibroblasts; DNA content; analytical purpose; biological parameters; dark signal; digital imaging fluorescence microscopy; efficiency; fluorescence intensities; intensified TV cameras; living cells; low light level intensity; nuclear fluorescence intensities; numerical analysis; pixel-to-pixel response variations; protocol; Biomedical imaging; Cameras; Digital images; Electrons; Fluorescence; Glass; Image analysis; Microscopy; Pixel; Probes;
Conference_Titel :
Engineering in Medicine and Biology Society, 1989. Images of the Twenty-First Century., Proceedings of the Annual International Conference of the IEEE Engineering in
Conference_Location :
Seattle, WA
DOI :
10.1109/IEMBS.1989.96105
