Title :
Fabrication and characteristics of bioFET albumin sensor using new self-assembled monolayer
Author :
Park, K.Y. ; Park, K.M. ; Cho, Y.S. ; Choi, S.Y. ; Kim, H.S. ; Bae, Y.S.
Author_Institution :
Sch. of Electr. Eng. & Comput. Sci., Kyungpook Nat. Univ., Daegu, South Korea
Abstract :
The BioFET albumin sensor with the gold gate as a gate material was fabricated and its characteristics were investigated. To enable label-free molecular detection, the BioFET gate surface was chemically modified by new immobilized molecular receptors, so called self-assembled monolayer (SAM) synthesized by a thiazole benzo crown ether ethylamine (TBECA)-thioctic acid to bind albumin-antibody. In this paper an albumin antigen-antibody binding generated above the gate surface was used as an albumin sensing mechanism of the BioFET. So the drain current was varied by antigen-antibody interactions on gate surface because the albumins have a negative charge. Experimental results related to the formation of SAM, antibody, and antigen were obtained from quartz crystal microbalance and surface plasmon resonance.
Keywords :
biosensors; field effect transistors; monolayers; self-assembly; Au; albumin antigen-antibody binding; albumin sensor; antigen-antibody interactions; bioFET; gate material; gate surface; immobilized molecular receptors; label-free molecular detection; quartz crystal microbalance; self-assembled monolayer; surface plasmon resonance; thiazole benzo crown ether ethylamine-thioctic acid; Biochemistry; Biological materials; Biological processes; Biosensors; Chemical and biological sensors; Fabrication; Gold; Proteins; Sensor phenomena and characterization; Silicon;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems, 2005. Digest of Technical Papers. TRANSDUCERS '05. The 13th International Conference on
Print_ISBN :
0-7803-8994-8
DOI :
10.1109/SENSOR.2005.1497440
