Title :
The silicon microphysiometer: a biosensor that detects cell-affecting agents
Author_Institution :
Molecular Devices Corp., Menlo Park, CA, USA
Abstract :
The metabolic activity of living cells has been measured with the light-addressable potentiometric sensor (LAPS). Cells are confined to a flow chamber, one surface of which is the LAPS. The rate of acidification of the culture medium (due to the production of acidic metabolites) is a measure of the cellular catabolic rate and can be measured repeatedly and nondestructively. This rate is sensitive to a wide variety of biological effector molecules and toxicants
Keywords :
biological techniques and instruments; biosensors; cellular biophysics; chemical variables measurement; silicon; Si microphysiometer; acidic metabolites; acidification; biological effector molecules; biosensor; cell-affecting agents; cellular catabolic rate; culture medium; flow chamber; light-addressable potentiometric sensor; living cells; metabolic activity; toxicants; Biomembranes; Biosensors; Cells (biology); Extracellular; Humans; Instruments; Production; Silicon; Testing; Toxicology;
Conference_Titel :
Engineering in Medicine and Biology Society, 1989. Images of the Twenty-First Century., Proceedings of the Annual International Conference of the IEEE Engineering in
Conference_Location :
Seattle, WA
DOI :
10.1109/IEMBS.1989.96111
