DocumentCode :
1809361
Title :
Poster title (mass spectrometric protein profiling analyses of pathological and physiological hypertrophy cardiac muscle tissues)
Author :
Kwon, Hyekyeong ; Chun, Jang-Soo ; Park, Zee-Yong ; Kim, Dong-Yu
Author_Institution :
Sch. of Life Sci., Gwangju Inst. of Sci. & Technol., Gwangju, South Korea
fYear :
2012
fDate :
23-25 Feb. 2012
Firstpage :
1
Lastpage :
1
Abstract :
The mammalian heart is a muscular organ that can adjust dynamic alterations in its workload. In response to physiological or pathological stimuli and development, the heart changes hypertrophic enlargement which increase in the size of individual cardiac myocytes. A comparative proteomic survey is needed to acquire systemic insights into mammalian cardiac hypertrophy. In this study, using a shotgun proteomics approach based on MudPIT and tandem mass spectrometry, we have gained global protein profiling for each of pathological and physiological cardiac hypertrophy models and applied a statistical method by normalization of spectral counts. We analyzed relative protein expression abundance and studied their biological functions with bioinformatics tools. Interestingly, between physiological and pathological cardiac hypertrophy there were little overlap in the expression profiles. More specifically, there was a significant, negative correlation in biological functions of two models. Thus, different models of pathological and physiological cardiac hypertrophy are associated with distinct proteins controlled each signaling pathway. These data demonstrate key biological functions which describe each type of cardiac hypertrophy.
Keywords :
bioinformatics; biomedical measurement; cardiology; mass spectroscopy; medical computing; molecular biophysics; muscle; proteins; proteomics; MudPIT; bioinformatics tools; global protein profiling; mass spectrometric protein profiling analysis; pathological hypertrophy cardiac muscle tissue; physiological cardiac hypertrophy model; physiological hypertrophy cardiac muscle tissue; protein expression; shotgun proteomics approach; tandem mass spectrometry; Biological system modeling; Mass spectroscopy; Pathology; Physiology; Proteins; Proteomics; Biological function; Cardiac hypertrophy; Comparative proteomics; Geneset analysis; KEGG; MudPIT; TAC mouse; Tandem mass spectrometry;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Computational Advances in Bio and Medical Sciences (ICCABS), 2012 IEEE 2nd International Conference on
Conference_Location :
Las Vegas, NV
Print_ISBN :
978-1-4673-1320-9
Electronic_ISBN :
978-1-4673-1319-3
Type :
conf
DOI :
10.1109/ICCABS.2012.6182652
Filename :
6182652
Link To Document :
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