Analysis of stretch-activated ion channels

Author

Akay, Metin ; Redling, Judy ; Craelius, William

Author_Institution

Dept. of Biomed. Eng., Rutgers Univ., Piscataway, NJ, USA

fYear

1992

fDate

1992

Firstpage

3

Lastpage

4

Abstract

Stretch-activated ion channels (SACs) in myocytes isolated from neonatal rats were studied in cell-attached patches. Stretch was applied to cell-attached patches by suction (5-20 mm Hg) in the recording pipet. Stretch caused the triggering of action potentials that were recorded in cell-attached patches as biphasic action currents (ACs). The significance of a temporal correlation between SAC open probability and ACs was tested using the Kolmogorov-Smirnov and Poisson distributions. It was shown that current from a small number of SACs injects sufficient charge (0.22 pC during 50 ms) to trigger action potentials in myocytes.

Keywords

bioelectric phenomena; cellular transport and dynamics; muscle; 5 to 20 mmHg; 50 ms; Kolmogorov-Smirnov distribution; Poisson distribution; action potentials triggering; biphasic action currents; cell-attached patches; myocytes; neonatal rats; open probability; stretch-activated ion channels; suction; temporal correlation; Biomedical engineering; Biomembranes; Distribution functions; Feedback; Kinetic theory; Mercury (metals); Pediatrics; Rats; Testing; Voltage;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioengineering Conference, 1992., Proceedings of the 1992 Eighteenth IEEE Annual Northeast

Conference_Location

Kingston, RI, USA

Print_ISBN

0-7803-0902-2

Type

conf

DOI

10.1109/NEBC.1992.285937

Filename

285937