Title :
Study on production of mycophenolic acid by Penicillium pinophilum using response surface metodology
Author :
Ramos-Ponce, L.M. ; Contreras-Esquivel, J.C. ; Saenz, Julio Montañez ; Lara-Cisneros, G. ; Garza-García, Y.
Author_Institution :
Dept. of Biotechnol., Univ. Autonomous of Coahuila, Coahuila, Mexico
Abstract :
Mycophenolic acid (MPA) can be produced from Penicillium pinophilum by submerged fermentation. Here we reported the use of the Plackett-Burman design to screen the limiting components for MPA production and subsequent use the response surface methodology was employed to derive a statistical model for media optimization towards production of MPA. The results obtained of Plackett-Burman indicated limitations of three components in the basal medium, aspartic acid, methionine and Tween 80. The determinant coefficient was 0.80, ensuring a satisfactory adjustment of the model to the experimental values. This statistical design was good in improving the titer of MPA up 923 mg/L and a specific productivity of 0.05 mg AMP/mg of biomass/h after 10 day of fermentation.
Keywords :
biological organs; biological tissues; biomedical materials; cellular biophysics; drugs; microorganisms; organic compounds; prosthetics; response surface methodology; Penicillium pinophilum; Plackett-Burman design; Tween 80; determinant coefficient; fermentation; limiting components; media optimization; methionine; mycophenolic acid production; response surface metodology; satisfactory adjustment; statistical design; statistical model; submerged fermentation; Biomass; Educational institutions; Mathematical model; Productivity; Response surface methodology; Strain; Plackett Burman; Response surface experiment; canonical analysis; sumerged fermentation;
Conference_Titel :
Bioengineering (ENBENG), 2012 IEEE 2nd Portuguese Meeting in
Conference_Location :
Coimbra
Print_ISBN :
978-1-4673-4524-8
DOI :
10.1109/ENBENG.2012.6331348
