Title :
Photothermal effect in imaging of the cells
Author :
Lapotko, D.O. ; Kuchinsky, G.S.
Author_Institution :
Lykov Heat & Mass Transfer Inst., Minsk, Byelorussia
Abstract :
The photothermal (PT) effect is applied for imaging and quantitative studies of samples with low optical contrast or low absorbance. The dual pulsed laser setup combined with phase-contrast illumination of a sample by probe laser provides visualisation of temperature field or absorbing structure of a sample. The spatial resolution is about 0.5 μm. The sensitivity of the PT technique provides imaging of live (non-stained) white blood cells (WBCs). An automated system and software was developed for optical and PT image acquisition, processing and analysis at the rate 1 cell per second. The system was tested during normal/abnormal blood cells (red blood cells, WBCs) studies
Keywords :
biothermics; blood; cellular biophysics; laser applications in medicine; photothermal effects; abnormal blood cells; absorbing structure; automated system; dual pulsed laser setup; live nonstained cells; normal blood cells; optical diagnostic technique; phase-contrast illumination; photothermal effect cell imaging technique; probe laser; red blood cells; temperature field visualisation; white blood cells; Lighting; Optical imaging; Optical pulses; Optical sensors; Photothermal effects; Probes; Spatial resolution; Temperature sensors; Visualization; White blood cells;
Conference_Titel :
Engineering in Medicine and Biology Society, 1994. Engineering Advances: New Opportunities for Biomedical Engineers. Proceedings of the 16th Annual International Conference of the IEEE
Conference_Location :
Baltimore, MD
Print_ISBN :
0-7803-2050-6
DOI :
10.1109/IEMBS.1994.411846
