Photothermal effect in imaging of the cells

Author

Lapotko, D.O. ; Kuchinsky, G.S.

Author_Institution

Lykov Heat & Mass Transfer Inst., Minsk, Byelorussia

fYear

1994

fDate

3-6 Nov 1994

Firstpage

653

Abstract

The photothermal (PT) effect is applied for imaging and quantitative studies of samples with low optical contrast or low absorbance. The dual pulsed laser setup combined with phase-contrast illumination of a sample by probe laser provides visualisation of temperature field or absorbing structure of a sample. The spatial resolution is about 0.5 μm. The sensitivity of the PT technique provides imaging of live (non-stained) white blood cells (WBCs). An automated system and software was developed for optical and PT image acquisition, processing and analysis at the rate 1 cell per second. The system was tested during normal/abnormal blood cells (red blood cells, WBCs) studies

Keywords

biothermics; blood; cellular biophysics; laser applications in medicine; photothermal effects; abnormal blood cells; absorbing structure; automated system; dual pulsed laser setup; live nonstained cells; normal blood cells; optical diagnostic technique; phase-contrast illumination; photothermal effect cell imaging technique; probe laser; red blood cells; temperature field visualisation; white blood cells; Lighting; Optical imaging; Optical pulses; Optical sensors; Photothermal effects; Probes; Spatial resolution; Temperature sensors; Visualization; White blood cells;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 1994. Engineering Advances: New Opportunities for Biomedical Engineers. Proceedings of the 16th Annual International Conference of the IEEE

Conference_Location

Baltimore, MD

Print_ISBN

0-7803-2050-6

Type

conf

DOI

10.1109/IEMBS.1994.411846

Filename

411846