DocumentCode :
1814476
Title :
High throughput microscopy: from images to data
Author :
Price, Jeffrey H.
Author_Institution :
Burnham Inst. for Medical Res., La Jolla, CA
fYear :
2006
fDate :
6-9 April 2006
Firstpage :
247
Lastpage :
250
Abstract :
For academic scientists, high throughput microscopy enables a paradigm shift from labor intensive qualitative microscopy to fast image-based quantitative biology. For pharmaceutical scientists, it enables replacing whole-well averaged readouts of simple cellular biomarkers with multiplexed cellular systems biological screens that include cell subpopulation and morphological characteristics. For research pathologists, the future looks bright for automating the process of reading and scoring tissue microarrays, which consist of a few hundred to a few thousand tissue sections on each slide. For image-based (or high content) screening, various algorithms have been developed to perform the automated cytometry needed to assess compound hits and/or dose responses. For many other drug screening and academic biological assays and for tissue microarrays, substantial challenges remain to be solved by more advanced image analysis techniques. For all of these applications, raw image data can comprise tens to hundreds of gigabytes (GB) per experiment and new frameworks enabling rapid algorithm development and validation are needed
Keywords :
biological tissues; biomedical optical imaging; cellular biophysics; medical image processing; optical microscopy; academic biological assays; automated cytometry; cell morphological characteristics; cell subpopulation; compound hits; dose responses; drug screening; fast image-based quantitative biology; high content screening; high throughput microscopy; image analysis; image-based screening; multiplexed cellular systems biological screens; pharmaceutical scientists; research pathologists; tissue microarrays; Blood; Cells (biology); Charge coupled devices; DNA; Image analysis; Image segmentation; Image sensors; Microscopy; Sensor arrays; Throughput;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Biomedical Imaging: Nano to Macro, 2006. 3rd IEEE International Symposium on
Conference_Location :
Arlington, VA
Print_ISBN :
0-7803-9576-X
Type :
conf
DOI :
10.1109/ISBI.2006.1624899
Filename :
1624899
Link To Document :
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