A shape analysis method to detect dendritic spine in 3D optical microscopy image

Author

Xu, Xiaoyin ; Cheng, Jie ; Witt, Rochelle M. ; Sabatini, Bernardo L. ; Wong, Stephen T C

Author_Institution

Harvard Center for Neurodegeneration & Repair, Harvard Med. Sch., Boston, MA

fYear

2006

fDate

6-9 April 2006

Firstpage

554

Lastpage

557

Abstract

Dendritic spines play an important role in synaptic transmission. Spines are small protrusions formed on the shafts of dendrites and they are manifest of many neurological conditions. Three dimensional images of dendrites and spines are obtained by confocal and two-photon laser scanning microscopy. We present an image processing method based on shape analysis to detect spines and separate spines from the dendrites for quantitative study. The method applies two grassfire procedures to first find the tips of spines and then reversely locate the boundary between spines and dendrites. The boundary is determined as the propagation front that undergoes the maximum change in plane area of the grassfire procedure. We use real data to demonstrate the performance of the method

Keywords

biomedical optical imaging; cellular biophysics; laser applications in medicine; medical image processing; neurophysiology; optical microscopy; two-photon processes; 3D optical microscopy image; confocal microscopy; dendritic spine detection; grassfire procedures; image processing; neurological conditions; shape analysis; synaptic transmission; two-photon laser scanning microscopy; Biomedical imaging; Deconvolution; Image analysis; Image processing; Image restoration; Image segmentation; Neurotransmitters; Optical microscopy; Shafts; Shape;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Imaging: Nano to Macro, 2006. 3rd IEEE International Symposium on

Conference_Location

Arlington, VA

Print_ISBN

0-7803-9576-X

Type

conf

DOI

10.1109/ISBI.2006.1624976

Filename

1624976