Title :
Target DNA recognition using electrochemical impedance spectroscopy
Author :
Michaels, Pauline ; Ciampi, Simone ; Yean, Chan Yean ; Gooding, J. Justin
Author_Institution :
Sch. of Chem., Univ. of New South Wales, Sydney, NSW, Australia
Abstract :
Electrochemical impedance spectroscopy was used to differentiate between different target DNA sequences without the use of a redox label or application of large bias voltages. This method relies on the formation of a mixed self-assembled monolayer (SAMs) composed of single-stranded DNA and a diluent, mercaptoethanol. Recognition of the DNA analytes was achieved through the significant increase in conductance for the double stranded DNA, as compared to single-stranded DNA. Non-complementary and single base-pair mismatches were also able to be differentiated.
Keywords :
DNA; biological techniques; electrochemical impedance spectroscopy; molecular biophysics; molecular configurations; monolayers; self-assembly; DNA analytes; DNA recognition; DNA sequences; double stranded DNA; electrochemical impedance spectroscopy; large bias voltages; mercaptoethanol; mixed self-assembled monolayer; redox label; single base-pair mismatches; single-stranded DNA; Biosensors; Capacitance; DNA; Electrochemical impedance spectroscopy; Electrodes; Gold; Probes; DNA; electrochemical impedance spectroscopy; gold-electrodes;
Conference_Titel :
Nanoscience and Nanotechnology (ICONN), 2010 International Conference on
Conference_Location :
Sydney, NSW
Print_ISBN :
978-1-4244-5261-3
Electronic_ISBN :
978-1-4244-5262-0
DOI :
10.1109/ICONN.2010.6045241
