Wide-field extrinsic optical signal imaging of fluorescence potassium sensors

Author

Pujari, R.A. ; Shitole, P.S. ; Charnani, V.S. ; McShane, M.J. ; Robinson, C.J.

Author_Institution

Biomedical Eng. Program, Louisiana State Univ., Ruston, LA

fYear

2006

fDate

6-9 April 2006

Firstpage

782

Lastpage

785

Abstract

Wide-field extrinsic optical signal (EOS) imaging is an excellent method for its spatial resolution of 50-100 mum and a wide imaging field of several mm that cannot be achieved by any microscope. Fluorescence images of a nanoengineered sensor for potassium, fabricated by using electrostatic layer-by-layer (LbL) self-assembly technique, were collected by a wide-field EOS imaging system under different concentrations of [K⁺]. The images were analyzed using MATLAB to study the quenching properties of the potassium nanosensor

Keywords

bioelectric phenomena; biomedical optical imaging; electric sensing devices; fluorescence; microsensors; nanotechnology; optical sensors; potassium; self-assembly; K; MATLAB; electrostatic layer-by-layer self-assembly; fluorescence images; fluorescence potassium sensors; nanoengineered sensor; quenching properties; wide-field extrinsic optical signal imaging; Earth Observing System; Electrostatics; Fluorescence; Image sensors; Optical imaging; Optical microscopy; Optical sensors; Self-assembly; Sensor systems; Spatial resolution;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Imaging: Nano to Macro, 2006. 3rd IEEE International Symposium on

Conference_Location

Arlington, VA

Print_ISBN

0-7803-9576-X

Type

conf

DOI

10.1109/ISBI.2006.1625033

Filename

1625033