Title :
Automation of the detection of lung cancer cells in minimal samples of bronchioalveolar lavage
Author :
Ortiz-de-Solorzano, Carlos ; Pengo, Thomas ; Galarraga, Miguel ; Barrutia, Arrate Munoz
Author_Institution :
Center for Appl. Med. Res., Univ. of Navarra, Pamplona
Abstract :
We present the hardware and software specification of a quantitative, multidimensional and multispectral microscopy system designed for the detection of lung cancer using minimal samples of bronchoalveolar lavage (BAL). BAL samples were stained using FICTION: Fluorescence Immunophenotyping and Interphase Cytogenetics as a Tool for the Investigation of Neoplasms. Our system allows preliminary immunophenotypic detection of rare cancerous candidate cells, followed by accurate three-dimensional analysis of genomic integrity, to confirm or refute the initial assessment. Our results show that our automated analysis can accurately assist a human expert in the diagnostic evaluation of BAL samples.
Keywords :
cancer; lung; medical diagnostic computing; microscopy; patient diagnosis; FICTION method; bronchioalveolar lavage; detection automation; diagnostic evaluation; immunophenotypic detection; lung cancer cells; microscopy system; Automation; Cancer detection; Fluorescence; Genomics; Hardware; Immune system; Lungs; Microscopy; Multidimensional systems; Neoplasms;
Conference_Titel :
Biomedical Imaging: From Nano to Macro, 2008. ISBI 2008. 5th IEEE International Symposium on
Conference_Location :
Paris
Print_ISBN :
978-1-4244-2002-5
Electronic_ISBN :
978-1-4244-2003-2
DOI :
10.1109/ISBI.2008.4540995
