Title :
Multiple-compartment chip for parallel recordings of epileptic activity from organotypic cultures
Author :
Berdichevsky, Y. ; Staley, K.J.
Author_Institution :
Med. Sch., Massachusetts Gen. Hosp., Dept. of Neurology, Harvard Univ., Boston, MA, USA
fDate :
April 27 2011-May 1 2011
Abstract :
Organotypic hippocampal slice culture is an in vitro model of post-traumatic epilepsy. To increase experimental throughput for development of anti-epileptic drugs, we designed a system for parallel recordings of neural activity from several cultures. We recorded spontaneous electrical activity from four organotypic cultures on the same recording chip for more than seven weeks. We confirmed that hippocampal cultures develop spontaneous epileptiform activity. Cultures exhibited a quiet latent period followed by interictal and ictal epileptiform activity.
Keywords :
bioMEMS; bioelectric phenomena; biomedical electrodes; biomedical measurement; brain; cellular biophysics; diseases; neurophysiology; antiepileptic drug development; epileptic activity parallel recordings; ictal epileptiform activity; interictal epileptiform activity; multiple compartment chip; neural activity parallel recordings; organotypic cultures; organotypic hippocampal slice culture; post traumatic epilepsy in vitro model; recording chip; spontaneous electrical activity; spontaneous epileptiform activity; Drugs; Electrodes; Epilepsy; In vitro; Injuries; Neurons; Noise;
Conference_Titel :
Neural Engineering (NER), 2011 5th International IEEE/EMBS Conference on
Conference_Location :
Cancun
Print_ISBN :
978-1-4244-4140-2
DOI :
10.1109/NER.2011.5910613
