3D imaging of microscopic structures using a proton beam

Author

Michelet-Habchi, C. ; Incerti, S. ; Aguer, P. ; Barberet, Ph. ; Gontier, E. ; Guinefolleau, T. ; Moretto, Ph. ; Pouthier, A. ; Pouthier, T. ; Smith, R.W.

Author_Institution

Centre d´´Etudes Nucleates de Bordeaux, Bordeaux I Univ., Gradignan, France

Volume

3

fYear

2003

fDate

19-25 Oct. 2003

Firstpage

1976

Abstract

The use of ion beams a few micrometers in diameter as a tomographic probe could constitute a powerful tool for displaying the 3D structure of samples a few tens or hundreds of micrometers thick in a non-destructive way. At CENBG, ion beam micro-tomography has been developed for biomedical applications at the cell level. The combination of STIM and computed tomography gives access to the 3D distribution of density (in g/cm³) within the analysed volume. The aim is to explore intracellular micro-structures, the sole preparation required being freeze-drying permitting analysis under vacuum.

Keywords

cellular biophysics; computerised tomography; ion microscopy; proton beams; 3D imaging; CENBG; biomedical applications; computed tomography; freeze drying; intracellular microstructures; ion beam microtomography; microscopic structures; proton beam; scanning transmission ion microscopy; Collimators; Computed tomography; Energy loss; Helium; Imaging phantoms; Ion beams; Optical microscopy; Particle beams; Probes; Visualization;

fLanguage

English

Publisher

ieee

Conference_Titel

Nuclear Science Symposium Conference Record, 2003 IEEE

ISSN

1082-3654

Print_ISBN

0-7803-8257-9

Type

conf

DOI

10.1109/NSSMIC.2003.1352267

Filename

1352267