Micro-rotation imaging deconvolution

Recently, micro-rotation confocal microscopy has enabled the acquisition of a sequence of slices of non adherent living cells obtained during a partially controlled rotation movement of the cell through the focal plane. Although we are now able to estimate the 3D position of every slice with respect to the frame, the reconstruction of the cell from the positioned slices remains a problem that this paper address. In our context, 3D spatially-varying PSF and missing data are the two main particularities of this problem. Experiments illustrate the ability of the classical EM algorithm to deconvolve efficiently cell volume and also to deal with missing data.