Title :
Membrane electronics of reconstituted thermophilic proteins: polymerization of planar phospholipid bilayer membranes
Author :
Hamamoto, Toshiro ; Hirata, Hajime ; Kagawa, Yasuo
Author_Institution :
Dept. of Biochem., Jichi Med. Sch., Tochigi, Japan
Abstract :
Stable phospholipid bilayer membranes were made by polymerizing the phospholipid 1,2-di(2,4-octadecadienoyl)-3-phosphatidylcholine (DODPC) by means of UV irradiation and used to measure proton translocation and ATP (adenosine triphosphate) synthesis by H+-ATPase from the thermophilic bacterium PS3. The polymerized membrane was much more tight and stable than the monomeric DODPC membrane, but not more stable than the soybean phospholipid bilayer membrane previously used for reconstruction of H+-ATPase
Keywords :
biomembranes; biothermics; molecular biophysics; proteins; 1,2-di(2,4-octadecadienoyl)-3-phosphatidylcholine; ATP synthesis; PS3; UV irradiation; membrane electronics; membrane polymerization; planar phospholipid bilayer membranes; proton translocation; reconstituted thermophilic proteins; thermophilic bacterium; Biochemistry; Biomembranes; Capacitance; Current measurement; Electric variables measurement; Electrons; Lipidomics; Polymers; Proteins; Protons;
Conference_Titel :
Engineering in Medicine and Biology Society, 1989. Images of the Twenty-First Century., Proceedings of the Annual International Conference of the IEEE Engineering in
Conference_Location :
Seattle, WA
DOI :
10.1109/IEMBS.1989.96228
