Membrane electronics of reconstituted thermophilic proteins: polymerization of planar phospholipid bilayer membranes

Author

Hamamoto, Toshiro ; Hirata, Hajime ; Kagawa, Yasuo

Author_Institution

Dept. of Biochem., Jichi Med. Sch., Tochigi, Japan

fYear

1989

fDate

9-12 Nov 1989

Abstract

Stable phospholipid bilayer membranes were made by polymerizing the phospholipid 1,2-di(2,4-octadecadienoyl)-3-phosphatidylcholine (DODPC) by means of UV irradiation and used to measure proton translocation and ATP (adenosine triphosphate) synthesis by H⁺-ATPase from the thermophilic bacterium PS3. The polymerized membrane was much more tight and stable than the monomeric DODPC membrane, but not more stable than the soybean phospholipid bilayer membrane previously used for reconstruction of H⁺-ATPase

Keywords

biomembranes; biothermics; molecular biophysics; proteins; 1,2-di(2,4-octadecadienoyl)-3-phosphatidylcholine; ATP synthesis; PS3; UV irradiation; membrane electronics; membrane polymerization; planar phospholipid bilayer membranes; proton translocation; reconstituted thermophilic proteins; thermophilic bacterium; Biochemistry; Biomembranes; Capacitance; Current measurement; Electric variables measurement; Electrons; Lipidomics; Polymers; Proteins; Protons;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 1989. Images of the Twenty-First Century., Proceedings of the Annual International Conference of the IEEE Engineering in

Conference_Location

Seattle, WA

Type

conf

DOI

10.1109/IEMBS.1989.96228

Filename

96228