Study of the inducement of PTFE electret on apoptosis of MKN tumor cell

Author

Jian, Jiang ; Chengrong, Song ; Lili, Cui ; Zhongfu, XIA

Author_Institution

Dept. of Phys. & Math., Second Mil. Med. Univ., Shanghai, China

fYear

1999

fDate

1999

Firstpage

225

Lastpage

227

Abstract

By using the electrostatics field and microcurrent which was produced by an electret to induce the apoptosis process, we applied a ±300 V PTFE electret on the MKN tumor cell for 24, 48 and 72 h, respectively, to study the influence of electret on apoptosis by means of flow cytometry. It is shown that 1) compared with the control group, after 24 h action of -300 V PTFE on MKN tumor cell, the apoptosis amount of MKN tumor cell increased from 1% to 6% (some even to 15%) and intracellular calcium (Ca⁺²) concentration elevated, which indicated that the negative electret can enhance apoptosis;. 2) when +300 V PTFE was applied to MKN tumor cell for 24 h, it could restrain apoptosis; 3) with the increase of action time of electret on MKN tumor cell, the effect of induced apoptosis is proportional to the time

Keywords

bioelectric phenomena; biological effects of fields; cellular biophysics; electrets; patient treatment; polymers; tumours; 24 to 72 h; 300 V; Ca⁺²; MKN tumor cell; PTFE electret; apoptosis; electrostatic field; flow cytometry; induced apoptosis; intracellular calcium concentration; microcurrent; negative electret; Calcium; Cells (biology); DNA; Electrets; Electrostatics; Neoplasms; Sediments; Surface charging; Tumors; USA Councils;

fLanguage

English

Publisher

ieee

Conference_Titel

Electrets, 1999. ISE 10. Proceedings. 10th International Symposium on

Conference_Location

Athens

Print_ISBN

0-7803-5025-1

Type

conf

DOI

10.1109/ISE.1999.831984

Filename

831984