Title :
Sensors on surfaces
Author :
Schoen, P.E. ; Fare, T. ; Bhatia, S.K. ; Shriver-Lake, L.C. ; Ligler, F.S.
Author_Institution :
US Naval Res. Lab., Washington, DC, USA
Abstract :
Immobilization of the enzyme acetylcholinesterase on a glass substrate has been demonstrated. The substrate was prepared with a thiol-terminal silane, to which was bonded the heterobifunctional cross-linker, N-gamma-maleimidobutyryloxy succinimide ester. The terminal amino groups of the enzyme were then attached to the cross-linker, yielding a surface enzyme coverage of 0.46 ng/mm2 with approximately 40% of the activity of the bound enzymes retained. Strategies for fabrication of practical biosensors are discussed
Keywords :
biosensors; proteins; N-gamma-maleimidobutyryloxy succinimide ester; acetylcholinesterase; biosensors fabrication; glass substrate; heterobifunctional cross-linker; immobilized enzyme; surface enzyme coverage; thiol-terminal silane; Biochemical analysis; Biochemistry; Biosensors; Bonding; Detectors; Differential amplifiers; Electrodes; Glass; Proteins; Substrates;
Conference_Titel :
Engineering in Medicine and Biology Society, 1989. Images of the Twenty-First Century., Proceedings of the Annual International Conference of the IEEE Engineering in
Conference_Location :
Seattle, WA
DOI :
10.1109/IEMBS.1989.96250
