Use of silicon and GaAs pixel detectors for digital autoradiography

Author

Bertolucci, E. ; Conti, M. ; Grossi, G. ; Mancini, E. ; Russo, P. ; Campbell, M. ; Chesi, E. ; Heijne, E. ; Middelkamp, P. ; Scharfetter, L. ; Randaccio, P. ; Beccherle, R. ; Guerra, A. Del ; Gambaccini, M. ; Marchesini, R. ; Via, C. Da ; D´Auria, S. ; O´

Author_Institution

Ist. Nazionale di Fisica Nucl., Napoli, Italy

Volume

2

fYear

1996

fDate

2-9 Nov. 1996

Firstpage

835

Abstract

Solid state detectors made of Si (4.8/spl times/8 mm/sup 2/) and GaAs (6.4/spl times/8 mm/sup 2/) pixel matrices bump-bonded to the Omega2 and Omega3 electronic read-out systems, developed at CERN for H.E.P. experiments, have been used to obtain autoradiographic images of clusters of human epithelial cells and DNA fragments separated via electrophoresis, both labeled with /sup 32/P. The system has shown a good minimum detectable activity per unit area of 2.10/sup -4/ cps mm/sup -2/, and has proved linear for count rate in the range 0.2-20 cps, typical of autoradiography. The pixel dimensions are 75/spl times/500 /spl mu/m/sup 2/ (Si-Omega2) and 50/spl times/500 /spl mu/m/sup 2/(GaAs-Omega3), respectively. We are able to clearly localize clusters of cells which have incorporated the radioactive tracer and DNA fragments on an electrophoretic gel on paper (blots).

Keywords

DNA; X-ray detection; X-ray imaging; biomedical equipment; cellular biophysics; position sensitive particle detectors; radiation therapy; radioisotope imaging; semiconductor counters; silicon radiation detectors; /sup 32/P; 4.8 mm; 50 mum; 500 mum; 6.4 mm; 75 mum; 8 mm; DNA fragments; GaAs pixel detectors; Omega2 electronic read-out system; Omega3 electronic read-out system; Si pixel detectors; digital autoradiography; electrophoretic gel; human epithelial cells; minimum detectable activity; solid state detectors; Biomedical imaging; DNA; Detectors; Gallium arsenide; Humans; Phosphors; Pixel; Silicon; Solid state circuits; Spatial resolution;

fLanguage

English

Publisher

ieee

Conference_Titel

Nuclear Science Symposium, 1996. Conference Record., 1996 IEEE

Conference_Location

Anaheim, CA, USA

ISSN

1082-3654

Print_ISBN

0-7803-3534-1

Type

conf

DOI

10.1109/NSSMIC.1996.591467

Filename

591467