DocumentCode :
1834303
Title :
Effect of self-assembled monolayers (SAMs) in binding glucose oxidase for electro-enzymatic glucose sensor with gold electrodes
Author :
Patel, J.N. ; Kaminska, Bozena ; Gray, B. ; Gates, B.D.
Author_Institution :
Simon Fraser Univ., Burnaby
fYear :
2007
fDate :
22-26 Aug. 2007
Firstpage :
2677
Lastpage :
2680
Abstract :
In this paper, a new approach to improve long term stability of electro-enzymatic glucose sensors is presented. Self-assembled monolayers (SAMs) are employed for surface treatment of the gold electrodes to improve adhesion. Three types of functional alkylthiols, namely 11-amino-1-undecanethiol hydrochloride, 1-hexadecanethiol (1- hexadecanthiol) and 1,9-nonanedithiol, and bovine serum albumin (BSA) are investigated in our study. Alkylhiols are used for surface treatment of gold electrodes and BSA is mixed in enzyme (glucose oxidase) solution to realize the proposed sensors. Furthermore, gold is investigated as an electrode material. Both reference and active electrodes are fabricated using only gold. The current response of the modified sensors showed long term stability. However, the unmodified sensors showed very short term stability because glucose oxidase does not adhere well to the electrodes. The proposed modified sensors also show lower drift than the unmodified sensors without surface treatment and BSA.
Keywords :
adhesion; biosensors; chemical sensors; electrochemical electrodes; enzymes; gold; monolayers; self-assembly; sugar; surface treatment; 1,9-nonanedithiol; 1-hexadecanethiol (1- hexadecanthiol); 11-amino-1-undecanethiol hydrochloride; Au; BSA; SAM; binding glucose oxidase; bovine serum albumin; electroenzymatic glucose sensor; functional alkylthiols; glucose oxidase enzyme solution; gold electrode adhesion; gold electrode surface treatment; gold electrodes; long term sensor stability; self assembled monolayers; Adhesives; Biochemistry; Biosensors; Diabetes; Electrodes; Fabrication; Gold; Stability; Sugar; Surface treatment; Alkanes; Biosensing Techniques; Electrodes; Enzymes, Immobilized; Glucose Oxidase; Gold; Serum Albumin, Bovine; Sulfhydryl Compounds;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Engineering in Medicine and Biology Society, 2007. EMBS 2007. 29th Annual International Conference of the IEEE
Conference_Location :
Lyon
ISSN :
1557-170X
Print_ISBN :
978-1-4244-0787-3
Type :
conf
DOI :
10.1109/IEMBS.2007.4352880
Filename :
4352880
Link To Document :
بازگشت