Title :
Chromium hexacyanoferrate modified biosensor based on PQQ-dependent glucose dehydrogenase
Author :
Ta-Feng Tseng ; Yang-Li Yang ; Shyh-Liang Lou
Author_Institution :
Chung-Yuan Christian Univ., Chung-Li
Abstract :
A pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase based platinum electrode was developed to detect glucose. Chromium hexacyanoferrate was modified onto this electrode to serve as an electron transfer mediator between PQQ and the platinum electrode. This biosensor showed the optimal response of glucose measurements at pH 7 and an operation potential of +0.4 V (vs. Ag/AgCl). More importantly the lifespan of the biosensor has been last for 47 days without significant enzyme activity degradation.
Keywords :
biomedical electrodes; biosensors; chromium compounds; enzymes; molecular biophysics; pH; PQQ-dependent glucose dehydrogenase; chromium hexacyanoferrate modified biosensor; electron transfer mediator; enzyme activity degradation; pH; platinum electrode; pyrroloquinoline quinone; Biochemistry; Biomedical engineering; Biomembranes; Biosensors; Chromium; Electrodes; Electrons; Humans; Platinum; Sugar; Biosensing Techniques; Chromium; Electron Transport; Ferrocyanides; Glucose; Glucose Dehydrogenases; PQQ Cofactor;
Conference_Titel :
Engineering in Medicine and Biology Society, 2007. EMBS 2007. 29th Annual International Conference of the IEEE
Conference_Location :
Lyon
Print_ISBN :
978-1-4244-0787-3
DOI :
10.1109/IEMBS.2007.4352881
