Chromium hexacyanoferrate modified biosensor based on PQQ-dependent glucose dehydrogenase

Author

Ta-Feng Tseng ; Yang-Li Yang ; Shyh-Liang Lou

Author_Institution

Chung-Yuan Christian Univ., Chung-Li

fYear

2007

fDate

22-26 Aug. 2007

Firstpage

2681

Lastpage

2684

Abstract

A pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase based platinum electrode was developed to detect glucose. Chromium hexacyanoferrate was modified onto this electrode to serve as an electron transfer mediator between PQQ and the platinum electrode. This biosensor showed the optimal response of glucose measurements at pH 7 and an operation potential of +0.4 V (vs. Ag/AgCl). More importantly the lifespan of the biosensor has been last for 47 days without significant enzyme activity degradation.

Keywords

biomedical electrodes; biosensors; chromium compounds; enzymes; molecular biophysics; pH; PQQ-dependent glucose dehydrogenase; chromium hexacyanoferrate modified biosensor; electron transfer mediator; enzyme activity degradation; pH; platinum electrode; pyrroloquinoline quinone; Biochemistry; Biomedical engineering; Biomembranes; Biosensors; Chromium; Electrodes; Electrons; Humans; Platinum; Sugar; Biosensing Techniques; Chromium; Electron Transport; Ferrocyanides; Glucose; Glucose Dehydrogenases; PQQ Cofactor;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2007. EMBS 2007. 29th Annual International Conference of the IEEE

Conference_Location

Lyon

ISSN

1557-170X

Print_ISBN

978-1-4244-0787-3

Type

conf

DOI

10.1109/IEMBS.2007.4352881

Filename

4352881