Title :
Implementation of Electrochemiluminescence Microanalysis in PCB Technology
Author :
Pittet, P. ; Guo-Neng Lu ; Galvan, J.-M. ; Ferrigno, R. ; Stephan, K. ; Blum, L.J. ; Leca-Bouvier, B.D.
Author_Institution :
Inst. des Nanotechnol. de Lyon, Villeurbanne
Abstract :
We present an instrumental development to implement electrochemiluminescence (ECL) microanalysis using printed circuit board (PCB) technology. PCB gold macro- (10 mm2) and micro- (0.09 mm2) electrodes and two ECL microfluidic devices are designed, fabricated and tested via luminol ECL detection. Potential modulation is performed between 0.7 and 0 V vs. Ag/AgCl for luminol oxidation, thus giving rise to on/off ECL responses in the presence of hydrogen peroxide. Synchronous detection is adopted to allow weak ECL signal recovery at a very low signal-to-noise ratio (SNR). The detection limit obtained with the two ECL microfluidic devices is 50 nM and 100 nM H2O2 for macroelectrodes and microelectrodes, respectively.
Keywords :
biochemistry; biomedical electrodes; chemical analysis; chemiluminescence; electroluminescence; microelectrodes; microfluidics; printed circuits; ECL microfluidic devices; PCB gold electrodes; PCB technology; electrochemiluminescence microanalysis; luminol ECL detection; luminol oxidation; macroelectrodes; microelectrodes; potential modulation; printed circuit board; weak ECL signal recovery; Chemical technology; Copper; Electrodes; Fabrication; Gold; Instruments; Interference; Microfluidics; Printed circuits; Substrates; Electrochemistry; Electronics; Hydrogen Peroxide; Luminescent Agents; Luminescent Measurements; Luminol; Microelectrodes; Microfluidic Analytical Techniques; Silver; Silver Compounds;
Conference_Titel :
Engineering in Medicine and Biology Society, 2007. EMBS 2007. 29th Annual International Conference of the IEEE
Conference_Location :
Lyon
Print_ISBN :
978-1-4244-0787-3
DOI :
10.1109/IEMBS.2007.4352946
