DocumentCode :
1839660
Title :
Carbon nanotubes-based electrochemical sensing for cell culture monitoring
Author :
Boero, Cristina ; Carrara, Sandro ; Del Vecchio, G. ; Albini, Giuseppe D. ; Calzà, Laura ; De Micheli, Giovanni
Author_Institution :
Ecole Polytech. Fed. de Lausanne, Lausanne, Switzerland
fYear :
2010
fDate :
13-15 July 2010
Firstpage :
288
Lastpage :
293
Abstract :
Monitoring of metabolic compounds, such as glucose and lactate, is extensively reported in literature, especially for clinical purposes. Instead, the application of such technologies for monitoring metabolites in cell cultures has not been explored. From one side, such devices can provide information to the current state-of-the-art of cell lines, particularly those which are not fully known, as stem and embryonic cells. On the other hand, those systems can pave the way to fully automation for growing cell cultures, when coupled with robots for feeding. Among different presented strategies to develop biosensors, carbon nanotubes exhibit great properties, particularly suitable for biosensing. In this work nanostructured electrodes by using multi-walled carbon nanotubes are presented for the detection of glucose and lactate. Firstly, some results from simulations are illustrated in order to foresee the behavior of carbon nanotubes depending on their orientation, when they are dispersed onto the electrode surface. Then, such developed biosensors are characterized in terms of sensitivity and detection limit, and are compared to previously published results. Finally, monitoring of a cell culture is performed and the behavior of metabolites is analyzed as biosensors validation.
Keywords :
biochemistry; biological techniques; biosensors; carbon nanotubes; cellular biophysics; electrochemical electrodes; electrochemical sensors; enzymes; molecular biophysics; nanosensors; C; biosensors; cell culture monitoring; electrochemical sensing; enzyme probe; glucose; lactate; metabolites; multi-walled carbon nanotubes; nanostructured electrodes; Biosensors; Electrodes;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Complex Medical Engineering (CME), 2010 IEEE/ICME International Conference on
Conference_Location :
Gold Coast, QLD
Print_ISBN :
978-1-4244-6841-6
Type :
conf
DOI :
10.1109/ICCME.2010.5558828
Filename :
5558828
Link To Document :
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