Title :
A cell culture system with better spatial and time resolution
Author :
Ou, Yu-Cheng ; Hsu, Chih-Wen ; Yang, Lung-Jieh ; Han, Hsieh-Cheng ; Chen, Chien-Yuan
Author_Institution :
Dept. of Mech. & Electro-Mech. Eng., Tamkang Univ., Tamsui, Taiwan
Abstract :
A new cell culture system is demonstrated in this paper. This system includes a culture incubator with a compact optical monitoring module and an electro-chemical impedance sensor (ECIS) chip with gelatin micro patterns attracting living cells selectively. We provided a platform with a shorter observation interval of 4 hours and a finer line width of electrodes of sensor chips than the prior art in this work. The in-situ impedance measurement on HeLa cells was performed and shown the better spatial and time resolution.
Keywords :
bioelectric phenomena; biological techniques; biosensors; cellular biophysics; electric impedance; electric impedance measurement; electrochemical sensors; gelatin; lab-on-a-chip; microsensors; molecular biophysics; optical sensors; HeLa cells; cell culture system; compact optical monitoring module; electrochemical impedance sensor chip; gelatin micropatterns; in-situ impedance measurement; spatial resolution; time resolution; Biomedical monitoring; Biomedical optical imaging; Integrated optics; Monitoring; Optical device fabrication; Optical imaging; Optical sensors; cell culture; gelatin; glutaraldehyde; impedance sensing; in-situ monitoring;
Conference_Titel :
Nano/Molecular Medicine and Engineering (NANOMED), 2009 IEEE International Conference on
Conference_Location :
Tainan
Print_ISBN :
978-1-4244-5528-7
DOI :
10.1109/NANOMED.2009.5559113
