Title :
Measurement of Temperature Differences between Micro-regions in Water Using Near-Infrared Spectroscopy
Author :
Kakuta, N. ; Ozaki, A. ; Fuguo Li ; Arimoto, H. ; Yamada, Y.
Author_Institution :
Kyushu Univ., Fukuoka
Abstract :
We have developed a method for measuring the temperature of micro-regions in aqueous solutions using near-infrared spectroscopy that enables us to measure the temperature of biological cells, tissues, and biochemical solutions in vitro. The measurement principle is based on the fact that the peak wavelength of the water absorption band with its center near 1450 nm shifts with changes in temperature. The measurement system, which consists of a biological microscope and two spectrophotometers, can measure respective absorbance spectra for two areas that are each 80 mum in diameter. We formed the temperature distribution in a 500-mum thick water film by heating an immersed Nichrome wire and measured the temperature difference between the two areas. The results of the measurement were compared to a calculated temperature distribution.
Keywords :
biochemistry; biological tissues; biomedical optical imaging; biothermics; cellular biophysics; infrared imaging; absorbance spectra; aqueous solution micro-regions; biochemical solutions; biological cells; biological microscope; biological tissues; immersed Nichrome wire; near-infrared spectroscopy; spectrophotometers; temperature differences; water absorption band; Absorption; Area measurement; Biological cells; Biological tissues; In vitro; Spectroscopy; Temperature distribution; Temperature measurement; Water heating; Wavelength measurement; Hot Temperature; Spectrophotometry, Infrared; Water;
Conference_Titel :
Engineering in Medicine and Biology Society, 2007. EMBS 2007. 29th Annual International Conference of the IEEE
Conference_Location :
Lyon
Print_ISBN :
978-1-4244-0787-3
DOI :
10.1109/IEMBS.2007.4353355