• DocumentCode
    1851113
  • Title

    Hydrodynamic single-cell trapping for cellular assays

  • Author

    Lin, C.M. ; Tseng, C.C. ; Chen, C.L. ; Wo, Andrew M.

  • Author_Institution
    Inst. of Appl. Mech., Nat. Taiwan Univ., Taipei, Taiwan
  • fYear
    2009
  • fDate
    21-25 June 2009
  • Firstpage
    612
  • Lastpage
    615
  • Abstract
    This paper reports a hydrodynamic single-cell trapping platform for cellular assays. This approach utilizes an oscillatory microplate (100 mum times 100 mum) via Lorentz force to generate microvortices for cell trapping. The microdevice can trap a single cell in suspended state, which should aid analysis of a single cell. Results obtained with this platform show a single Jurkat cell can be trapped and labeled on CD45 antibodies under real-time monitoring.
  • Keywords
    bioMEMS; cellular biophysics; hydrodynamics; micromechanical devices; CD45 antibody; Lorentz force; cellular assays; hydrodynamic single-cell trapping; microdevice; microvortices; oscillatory microplate; single Jurkat cell; Acoustic beams; Chromium; Electrodes; Hydrodynamics; Lab-on-a-chip; Lorentz covariance; Magnetic fields; Microchannel; Monitoring; Optical resonators; Lorentz force; Oscillatory plate; microfluidics; microvortices; single cell trapping;
  • fLanguage
    English
  • Publisher
    ieee
  • Conference_Titel
    Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International
  • Conference_Location
    Denver, CO
  • Print_ISBN
    978-1-4244-4190-7
  • Electronic_ISBN
    978-1-4244-4193-8
  • Type

    conf

  • DOI
    10.1109/SENSOR.2009.5285399
  • Filename
    5285399