Title :
Hydrodynamic single-cell trapping for cellular assays
Author :
Lin, C.M. ; Tseng, C.C. ; Chen, C.L. ; Wo, Andrew M.
Author_Institution :
Inst. of Appl. Mech., Nat. Taiwan Univ., Taipei, Taiwan
Abstract :
This paper reports a hydrodynamic single-cell trapping platform for cellular assays. This approach utilizes an oscillatory microplate (100 mum times 100 mum) via Lorentz force to generate microvortices for cell trapping. The microdevice can trap a single cell in suspended state, which should aid analysis of a single cell. Results obtained with this platform show a single Jurkat cell can be trapped and labeled on CD45 antibodies under real-time monitoring.
Keywords :
bioMEMS; cellular biophysics; hydrodynamics; micromechanical devices; CD45 antibody; Lorentz force; cellular assays; hydrodynamic single-cell trapping; microdevice; microvortices; oscillatory microplate; single Jurkat cell; Acoustic beams; Chromium; Electrodes; Hydrodynamics; Lab-on-a-chip; Lorentz covariance; Magnetic fields; Microchannel; Monitoring; Optical resonators; Lorentz force; Oscillatory plate; microfluidics; microvortices; single cell trapping;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International
Conference_Location :
Denver, CO
Print_ISBN :
978-1-4244-4190-7
Electronic_ISBN :
978-1-4244-4193-8
DOI :
10.1109/SENSOR.2009.5285399
