A selective release method using electrolytically generated bubbles for cell array applications

Author

Kurakazu, Tomoaki ; Kuribayashi, Kaori ; Tsuda, Yukiko ; Kimura, Hiroshi ; Fujii, Teruo ; Sakai, Yasuyuki ; Takeuchi, Shoji

Author_Institution

Inst. of Ind. Sci., Univ. of Tokyo, Tokyo, Japan

fYear

2009

fDate

21-25 June 2009

Firstpage

2362

Lastpage

2365

Abstract

This paper describes a method of selective release of particles/cells by bubbles generated by electrolysis. The particles/cells trapped into microchambers that were produced with SU-8 on the top of an electrode of indium tin oxide (ITO) patterned on glass. The bubbles of oxygen and chlorine (or hydrogen) were selectively generated in a target chamber by applying voltage through the electrodes. We successfully released a microbead (100 mum in diameter) confined in the microchamber. Since this method is gentle enough to maintain the cellular activity, we believe that this method will be useful for quantitative analysis of cells in a microarray.

Keywords

bubbles; cellular arrays; chlorine; electrodes; electrolysis; hydrogen; indium compounds; microfabrication; microfluidics; oxygen; photoresists; Cl; H; InSnO; O; SU-8 resist; SiO₂; cell array applications; electrolysis; electrolytically generated bubbles; indium tin oxide electrode; microarray; microbead; microchambers; selective release method; size 100 mum; trapped cells; trapped particles; Anodes; Electrochemical processes; Electrodes; Glass; Hydrogen; Indium tin oxide; Laser theory; Microfluidics; Optical sensors; Voltage; bubble; cell; electrolysis; microfluidic device; selective release;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International

Conference_Location

Denver, CO

Print_ISBN

978-1-4244-4190-7

Electronic_ISBN

978-1-4244-4193-8

Type

conf

DOI

10.1109/SENSOR.2009.5285447

Filename

5285447