Study of single-cell electroendocytosis using Bio-MEMS technology

Author

Lin, Ran ; Chang, Donald C. ; Lee, Yi-Kuen

Author_Institution

Bioeng. Grad. Program, Hong Kong Univ. of Sci. & Technol., Hong Kong, China

fYear

2009

fDate

21-25 June 2009

Firstpage

2366

Lastpage

2369

Abstract

In this work, a micro electroendocytosis (EED) device was fabricated by MEMS technology to investigate the EED of mammalian cells. The corresponding endocytotic vesicles at the single-cell level were visualized using digital fluorescence video microscopy. The EED process under different electrical and biological parameters was studied in detail. The critical EED electric field strength for the HeLa cells using FM4-64 dye was determined to be 341.9plusmn24.2 V/cm. Beyond the critical electric field, the EED efficiency increased dramatically without significantly reducing cell viability. In addition, the EED efficiency as a function of multiple electric parameters were readily obtained based on large-scale micro EED experiments.

Keywords

bioMEMS; bioelectric phenomena; biological effects of fields; biological techniques; cellular biophysics; fluorescence; optical microscopy; FM4-64 dye; HeLa cells; bioMEMS technology; critical EED electric field strength; digital fluorescence video microscopy; endocytotic vesicles; mammalian cell EED; micro-EED device; micro-electroendocytosis device; single cell electroendocytosis; Biomembranes; Fluorescence; Large-scale systems; Lipidomics; Micromechanical devices; Microscopy; Plasmas; Pulse generation; Radio access networks; USA Councils; Bio-MEMS; Electroendocytosis; FM4-64 dye; HeLa cell; electroporation; endocytotic vesicle;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International

Conference_Location

Denver, CO

Print_ISBN

978-1-4244-4190-7

Electronic_ISBN

978-1-4244-4193-8

Type

conf

DOI

10.1109/SENSOR.2009.5285448

Filename

5285448