Title :
Forming self-assembled cell arrays and measuring the oxygen consumption rate of a single live cell
Author :
Etzkorn, James R. ; McQuaide, Sarah C. ; Anderson, Judy B. ; Meldrum, Deirdre R. ; Parviz, Babak A.
Author_Institution :
Electr. Eng., Univ. of Washington, Seattle, WA, USA
Abstract :
We report a method for forming arrays of live single cells on a chip using polymer micro-traps made of SU8. We have studied the toxicity of the microfabricated structures and the associated environment for two cell lines. We also report a method for measuring the oxygen consumption rate of a single cell using optical interrogation of molecular oxygen sensors placed in micromachined micro-wells by temporarily sealing the cells in the micro-traps. The new techniques presented here add to the collection of tools available for performing ldquosingle-cellrdquo biology. A single-cell self-assembly yield of 61% was achieved with oxygen draw down rates of 0.83, 0.82, and 0.71 fmol/minute on three isolated live A549 cells.
Keywords :
bioMEMS; biological techniques; cellular biophysics; chemical sensors; micromachining; oxygen; A549 cells; SU8 polymer microtraps; cellular oxygen consumption rate measurement; live single cell arrays; microfabricated structure cytotoxicity; micromachined microwells; optical molecular oxygen sensors; self assembled cell array formation; single cell biology; Biomedical optical imaging; Biosensors; Fluid flow; Glass; Microfluidics; Optical sensors; Oxygen; Polymers; Self-assembly; Semiconductor device measurement; SU8 toxicity; Single-cell; microfluidics; oxygen measurement; self-assembly;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International
Conference_Location :
Denver, CO
Print_ISBN :
978-1-4244-4190-7
Electronic_ISBN :
978-1-4244-4193-8
DOI :
10.1109/SENSOR.2009.5285450
