Title :
Electric Impedance Assisted Micropipette Aspiration
Author :
Kovanen, K. ; Lukkari, M. ; Purmonen, S. ; Ylikomi, T. ; Viitanen, J. ; Kallio, P.J.
Author_Institution :
Tampere Univ. of Technol., Tampere
Abstract :
Micropipette aspiration is a technique to selectively isolate cells from cell cultures using suction pressure. Cells can selectively be isolated one by one from neighboring cells into the micropipette. This paper presents a novel micropipette aspiration system assisted by an impedance measurement system. Furthermore, a method to reduce the adhesion force at a single cell level for a gentler detachment of the cell from a cultivation surface and surrounding cell connections is proposed.
Keywords :
adhesion; bioelectric phenomena; biomechanics; biomedical measurement; cellular biophysics; electric impedance measurement; adhesion force; cell connection; cell culture; cell deformation; cell isolation; cultivation surface; electric impedance measurement system; micropipette aspiration system; single cell level; suction pressure; Adhesives; Automatic control; Automation; Control systems; Impedance measurement; Magnetic levitation; Mechanical factors; Medical control systems; Microinjection; Micromanipulators; Micropipette aspiration; adherent cells; impedance measurement; microinjection; micromanipulation; Cell Physiological Phenomena; Cell Separation; Cells, Cultured; Electric Impedance; Electrochemistry; Humans; Micromanipulation; Suction;
Conference_Titel :
Engineering in Medicine and Biology Society, 2007. EMBS 2007. 29th Annual International Conference of the IEEE
Conference_Location :
Lyon
Print_ISBN :
978-1-4244-0787-3
DOI :
10.1109/IEMBS.2007.4353671
