A micro-fabricated capillary electrophoresis chip for DNA pre-concentration and separation

Author

Kuo, Chen-Hua ; Wang, Jung-Hao ; Lee, Gwo-Bin

Author_Institution

Dept. of Eng. Sci., Nat. Cheng Kung Univ., Tainan, Taiwan

fYear

2009

fDate

21-25 June 2009

Firstpage

366

Lastpage

369

Abstract

A simple online DNA pre-concentration and separation method by using a micro capillary electrophoresis (CE) chip integrated with a normally-closed valve activated by pneumatic suction has been developed. The normally-closed valve with anionic surface charges forms a nanoscale channel only allows the passage of electric current but traps the DNA samples so that they can be pre-concentrated. Obvious fluorescent-labeled DNA concentration is observed at the pre-concentration area. The phiX174 DNA/HaeIII markers separation signals are significantly enhanced comparing with the results of a traditional cross-shaped micro CE chip. Therefore, successful DNA pre-concentration for signal enhancement and separation analysis can be performed by using this new micro CE chip.

Keywords

DNA; bioMEMS; biological specimen preparation; capillarity; electrophoresis; flow control; lab-on-a-chip; microchannel flow; valves; DNA preconcentration method; DNA separation method; anionic surface charge; fluorescent labeled DNA concentration; microcapillary electrophoresis chip; nanoscale channel; phiX174 DNA-HaeIII marker; pneumatic suction activated valve; Chip scale packaging; Current; DNA; Electric potential; Electrokinetics; Reservoirs; Strontium; Surface treatment; Valves; Voltage; DNA separation; Micro capillary electrophoresis; normally-closed valve; pre-concentration;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International

Conference_Location

Denver, CO

Print_ISBN

978-1-4244-4190-7

Electronic_ISBN

978-1-4244-4193-8

Type

conf

DOI

10.1109/SENSOR.2009.5285486

Filename

5285486