Title :
A micro-fabricated capillary electrophoresis chip for DNA pre-concentration and separation
Author :
Kuo, Chen-Hua ; Wang, Jung-Hao ; Lee, Gwo-Bin
Author_Institution :
Dept. of Eng. Sci., Nat. Cheng Kung Univ., Tainan, Taiwan
Abstract :
A simple online DNA pre-concentration and separation method by using a micro capillary electrophoresis (CE) chip integrated with a normally-closed valve activated by pneumatic suction has been developed. The normally-closed valve with anionic surface charges forms a nanoscale channel only allows the passage of electric current but traps the DNA samples so that they can be pre-concentrated. Obvious fluorescent-labeled DNA concentration is observed at the pre-concentration area. The phiX174 DNA/HaeIII markers separation signals are significantly enhanced comparing with the results of a traditional cross-shaped micro CE chip. Therefore, successful DNA pre-concentration for signal enhancement and separation analysis can be performed by using this new micro CE chip.
Keywords :
DNA; bioMEMS; biological specimen preparation; capillarity; electrophoresis; flow control; lab-on-a-chip; microchannel flow; valves; DNA preconcentration method; DNA separation method; anionic surface charge; fluorescent labeled DNA concentration; microcapillary electrophoresis chip; nanoscale channel; phiX174 DNA-HaeIII marker; pneumatic suction activated valve; Chip scale packaging; Current; DNA; Electric potential; Electrokinetics; Reservoirs; Strontium; Surface treatment; Valves; Voltage; DNA separation; Micro capillary electrophoresis; normally-closed valve; pre-concentration;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International
Conference_Location :
Denver, CO
Print_ISBN :
978-1-4244-4190-7
Electronic_ISBN :
978-1-4244-4193-8
DOI :
10.1109/SENSOR.2009.5285486
